Journal:

Article Title: Identification of astrocyte-expressed factors that modulate neural stem/progenitor cell differentiation

doi: 10.1089/scd.2006.15.407

Figure Lengend Snippet: (a) Sample correlation coefficient (cc) plots of signal intensity (representing gene expression levels) obtained by using MAS4.1 software (Affymetrix); Note that replicates (NBH1 vs. NBH2, ADS1 vs. ADS2) had similar gene expression profiles (high cc values), whereas samples from different tissues (NBH vs. ADS, NBH vs. ASF) had distinct gene expression profiles (low cc values); (b) A modified Venn Diagram showing numbers of genes that are unique or common among these astrocytes derived from different tissues; Among 5,190 genes that are “Present” in at least one astrocyte sample, 3,394 (65.4%) genes were shared by all four types of astrocytes, 914 (17.6%) genes were shared by 3 types of astrocytes, 537 (10.3%) genes were shared by two types of astrocytes, and 527 (10.2%) genes were only “Present” in one type of astrocyte; (c) Among the 3,394 common genes shared by the astrocytes, 3,231 genes are also shared with ASF.

Article Snippet: Blocking antibodies to IL-1β (R&D Systems Cat. #AF-501-NA) and IL-6 (R&D Systems, Cat. #AF-506) were used at 1μg/mL.

Techniques: Gene Expression, Software, Modification, Derivative Assay

Journal:

Article Title: Identification of astrocyte-expressed factors that modulate neural stem/progenitor cell differentiation

doi: 10.1089/scd.2006.15.407

Figure Lengend Snippet: Genes that are expressed at higher levels in neurogenesis-promoting astrocytes

Article Snippet: Blocking antibodies to IL-1β (R&D Systems Cat. #AF-501-NA) and IL-6 (R&D Systems, Cat. #AF-506) were used at 1μg/mL.

Techniques: Binding Assay, Virus

Journal:

Article Title: Identification of astrocyte-expressed factors that modulate neural stem/progenitor cell differentiation

doi: 10.1089/scd.2006.15.407

Figure Lengend Snippet: (a) IL-6; (b) IL-1β; (c) VCAM-1; and (d) IGFBP6. The relative mRNA levels of each candidate genes were obtained by normalizing to an internal control, GAPDH. PCR results were consistent with the results obtained by microarray analysis.*, p<0.05; **, p<0.01; ***, p<0.001 (unpaired t-test).

Article Snippet: Blocking antibodies to IL-1β (R&D Systems Cat. #AF-501-NA) and IL-6 (R&D Systems, Cat. #AF-506) were used at 1μg/mL.

Techniques: Control, Microarray

Journal:

Article Title: Identification of astrocyte-expressed factors that modulate neural stem/progenitor cell differentiation

doi: 10.1089/scd.2006.15.407

Figure Lengend Snippet: (a) Both NRG/ED (p<0.05, n=8) and IL-6 (P<0.05, n=14) increased NeuroD1 promoter activity in NSPCs; (b) IL-6 treatment increased GFAP promoter activity in NSPCs (p<0.05, n=6); (c) Both IL-1β and IL-6 promote the neuronal differentiation of NSPCs (p<0.01, n=7 for both) and such effect could be blocked by their specific blocking antibodies (p<0.05, n=3 for both); (d) Antibodies to IL-6 and IL-1β blocking the neurogenic effects of NBH astrocytes on co-cultured NSPCs, control un-treated NSPCs co-cultured with NBH astrocytes. (e) IL-6, but not IL-1β also increased GFAP+ astrocyte differentiation of NSPCs (p<0.05). *, p<0.05; **, p<0.01, ***, p<0.001 (unpaired, 2-tailed, student's t-test).

Article Snippet: Blocking antibodies to IL-1β (R&D Systems Cat. #AF-501-NA) and IL-6 (R&D Systems, Cat. #AF-506) were used at 1μg/mL.

Techniques: Activity Assay, Blocking Assay, Cell Culture, Control

Journal:

Article Title: Identification of astrocyte-expressed factors that modulate neural stem/progenitor cell differentiation

doi: 10.1089/scd.2006.15.407

Figure Lengend Snippet: (a) The combination of IL-1β, IL-6, VCAM1, IP-10, Cathepsin S, and TGFβ2 (Combo) enhanced NeuroD1 promoter activity in NSPCs, analyzed by luciferase activity assays (p<0.01, n=14); (b) Immunofluorescent images showing TuJI+ neurons (red) differentiated from NSPCs that were treated by IL-1β, IL-6, Combo and RA in the absence of FGF-2. Such fluorescent staining results were quantified using unbiased stereology quantification to generate data shown in Fig 4c and Fig 5d. (c) A Z-stack confocal image of a TuJI+ neurons differentiated from IL-6 treated NSPCs; (d) IL-1β, IL-6 and Combo could promote neuronal differentiation of NSPCs indicated by the percentage of TuJI+ neurons. Combo was significantly more potent than IL-1β and IL-6 alone. *, p<0.05; **, p<0.01, ***, p<0.001 (unpaired, 2-tailed, student's t-test).

Article Snippet: Blocking antibodies to IL-1β (R&D Systems Cat. #AF-501-NA) and IL-6 (R&D Systems, Cat. #AF-506) were used at 1μg/mL.

Techniques: Activity Assay, Luciferase, Staining

Journal: EBioMedicine

Article Title: The Notch ligand DNER regulates macrophage IFNγ release in chronic obstructive pulmonary disease

doi: 10.1016/j.ebiom.2019.03.054

Figure Lengend Snippet: Nuclear translocation of Notch1 and NFκB active subunits was abrogated in LPS treated BMDM from DNER deficient mice. (a) Representative Western blot of NICD1 protein levels in cytoplasmic and nuclear extracts of samples from WT and DNER deficient BMDM treated with LPS (1μg/ml) for 24 h and untreated (2 independent experiments, 2/3 biological replicates). (b) mRNA abundance of Notch receptors ( Notch1, Notch2 ) and ligands ( Delta4, Jag1 ) in the samples from (a). Two-way ANOVA, Tukey's multiple comparisons test, * P < 0.05, *** P < 0.001. Data shown mean values ± SD. (c) Cytoplasmic and nuclear RelB, c-Rel, p105, p50 and p65 from samples described in (a). (d) Representative Western blots of phosphorylated (p)-IKKα/β, IKKα and IKKβ from 15 min LPS (1μg/ml) treated WT and DNER deficient BMDM. 2 independent experiments and 2/3 biological replicates, n = 3 (e) Enrichment plot of NFkB signalling (GO:0051059) following GSEA analysis of the microarray data from WT M1 vs DNER deficient M1 BMDM.

Article Snippet: Primary antibodies: NICD1 (1:500, ab8925, Abcam), RelB (1:200, sc-226, Santa Cruz), cRel (1:100, sc-6955, Santa Cruz), p105/50 (1:100, sc-1190, Santa Cruz), p65 (1:500, sc-372, Santa Cruz), pIKKα/β (1:1000 #2697, Cell Signalling), IKKα (1:1000 #2682S, Cell Signalling), IKKβ (1:1000 clone D30C6, #8943, Cell Signalling).

Techniques: Translocation Assay, Western Blot, Microarray

Journal: Cell

Article Title: Gene Regulatory Programs Conferring Phenotypic Identities to Human NK Cells

doi: 10.1016/j.cell.2018.11.045

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: DX5 microbeads , Miltenyi , Cat#130–052-501.

Techniques: Microscopy, Recombinant, DNA Library Preparation, Isolation, Microarray, Knock-Out, Expressing, Software